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Towards the development of a chemical lure to improve the management of invading rat populations

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posted on 08.12.2021, 18:31 authored by Paske, Grace Laura

Introduced species, such as Rattus norvegicus and Rattus rattus,have contributed to the extinction of many native animals and plants in New Zealand(NZ). Current strategies exist to monitor, manage and eradicate pest species. However, these haven’t always been completely successful and tools to detect small or invading densities remain to be developed. One possible new method to address this problem is the application of chemical attractants (lures). Recently, a major urinary protein (MUP) has been shown in male miceto act as a sexual attractant. MUPs modulate the release of volatile attractants and have potential to act as attractants themselves. Our aim was to determine if a similar MUP(s) and associated volatiles are present in the urine of rats, with the prospect of creating a chemical lure to use in rat detection and eradication. Using Gas Chromatography/Mass Spectrometry, potential volatiles in rat urine have been identified. Analysis of rat urine by gel electrophoresis has shown MUPs present in both sexes. A 22.4 kDa MUP in Rattus norvegicushas been synthesised and expressed in E.coliusing recombinant DNA technology. Preliminary steps have been made towards the production of a MUP based on ship rat DNA sequence. Future behavioral trials are needed to investigate whether the synthesised protein, in the presence or absence of the urinary-derived volatiles, is a sexual attractant.

History

Copyright Date

01/01/2013

Date of Award

01/01/2013

Publisher

Te Herenga Waka—Victoria University of Wellington

Rights License

Author Retains Copyright

Degree Discipline

Biomedical Science

Degree Grantor

Te Herenga Waka—Victoria University of Wellington

Degree Level

Masters

Degree Name

Master of Biomedical Science

ANZSRC Type Of Activity code

970106 Expanding Knowledge in the Biological Sciences

Victoria University of Wellington Item Type

Awarded Research Masters Thesis

Language

en_NZ

Victoria University of Wellington School

School of Biological Sciences

Advisors

McNatty, Ken