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Revisiting marine bioprospecting of tropical Indonesian macroalgae from West Timor

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thesis
posted on 07.12.2021, 19:54 by Turupadang, Wem

Marine algae are an important and historically rich source of new marine-based natural products. This thesis describes the screening of 40 Indonesian macroalgal samples using liquid chromatography-mass spectrometry (LC-MS) based molecular networking, and the subsequent nuclear magnetic resonance (NMR)-guided isolation and structural elucidation of a 6-deoxy-6-aminoglycoglyrecolipid (60). Molecular networking was performed using LC-MS/MS data through the online Global Natural Product Social Molecular Networking (GNPS) platform directly from crude extracts. NMR spectroscopy-guided screening was also employed targeting unique peaks detected by 1H NMR to validate any hits from GNPS. Out of 40 macroalgae specimens collected from West Timor waters, six samples were prioritised by the molecular networking screening. Proton NMR revealed three specimens with significantly interesting peaks but only one specimen, Laurencia snackeyi was purified further, which yielded compound 60.

History

Copyright Date

01/01/2018

Date of Award

01/01/2018

Publisher

Te Herenga Waka—Victoria University of Wellington

Rights License

Author Retains Copyright

Degree Discipline

Chemistry

Degree Grantor

Te Herenga Waka—Victoria University of Wellington

Degree Level

Masters

Degree Name

Master of Science

ANZSRC Type Of Activity code

1 PURE BASIC RESEARCH

Victoria University of Wellington Item Type

Awarded Research Masters Thesis

Language

en_NZ

Victoria University of Wellington School

School of Chemical and Physical Sciences

Advisors

Keyzers, Rob