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Resistance to microtubule-stabilising agents following point mutation of human βI-tubulin

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posted on 15.11.2021, 07:37 by Rowe, Matthew R.

Marine environments represent a rich source of bioactive secondary metabolites that may be harnessed for use in a therapeutic context. Two novel compounds, peloruside A and laulimalide, isolated from the marine sponges Mycale hentsheli and Cacospongia mycofijiensis, respectively, both demonstrate useful pharmacological properties in mammalian cells. These compounds share major similarities with microtubule-stabilising agents. Like other agents in this class, peloruside A and laulimalide bind to the β-tubulin subunit of microtubules, the primary cytoskeletal element of eukaryotic cells. These compounds enhance polymerisation dynamics between ternary microtubule structures and severely hinder necessary cytoskeletal rearrangements within the cell.  Over the course of a patient’s treatment, cancerous cells may develop multi-drug resistance phenotypes. P-glycoprotein drug efflux pumps play a major role in the development of therapy resistance in many cancers, as the current generation microtubule-stabilising agents are easily removed from diseased cells by upregulated efflux mechanisms. Unlike agents already in clinical application, both peloruside A and laulimalide are poor substrates for removal by these mechanisms, making them and their synthetic derivatives interesting as potential treatments for drug-resistant tumours.  Peloruside A and laulimalide exhibit potent nanomolar anti-mitotic activities in vitro and arrest cell cycle progression in G₂/M phase, leading to cell death – a characteristic mode of action among microtubule-stabilising agents. Unlike all known agents in this class, peloruside A and laulimalide share a secondary, unique binding region in β-tubulin. In the past decade our understanding of this region has developed, revealing a second, unique mechanism for stabilisation of microtubules.  Using mammalian cells to model physiological tubulin, the present study investigates the predicted role of aspartic acid 297 of human βI-tubulin in the binding association of both peloruside A and laulimalide. This particular amino acid is predicted to hydrogen bond with both compounds, contributing to their activity as stabilisers.  It was revealed that the introduction of a point mutation in D297 resulted in a small but highly consistent resistance phenotype to both compounds, but not to microtubule-stabilising agents that bind to the traditional, taxoid site on β-tubulin. It was concluded that aspartic acid 297 is likely to be one of the amino acids directly involved in the binding association of peloruside A and laulimalide to β-tubulin, contributing partial compound stabilisation. The rational synthesis of future analogues may benefit from these findings in the design of molecules with enhanced interactions at this particular amino acid residue.

History

Copyright Date

01/01/2015

Date of Award

01/01/2015

Publisher

Te Herenga Waka—Victoria University of Wellington

Rights License

Author Retains Copyright

Degree Discipline

Biotechnology

Degree Grantor

Te Herenga Waka—Victoria University of Wellington

Degree Level

Masters

Degree Name

Master of Science

ANZSRC Type Of Activity code

970106 Expanding Knowledge in the Biological Sciences

Victoria University of Wellington Item Type

Awarded Research Masters Thesis

Language

en_NZ

Victoria University of Wellington School

School of Biological Sciences

Advisors

Miller, John H.