Open Access Te Herenga Waka-Victoria University of Wellington
Browse

File(s) under embargo

2

month(s)

11

day(s)

until file(s) become available

Variability in the composition of porcine mucosal heparan sulfates

journal contribution
posted on 2023-06-27, 02:46 authored by L Sargison, RAA Smith, Susan CarnachanSusan Carnachan, Alison DainesAlison Daines, Amira Brackovic, Joel KidgellJoel Kidgell, V Nurcombe, SM Cool, Ian SimsIan Sims, Simon HinkleySimon Hinkley
Commercial porcine intestinal mucosal heparan sulfate (HS) is a valuable material for research into its biological functions. As it is usually produced as a side-stream of pharmaceutical heparin manufacture, its chemical composition may vary from batch to batch. We analysed the composition and structure of nine batches of HS from the same manufacturer. Statistical analysis of the disaccharide compositions placed these batches in three categories: group A had high GlcNAc and GlcNS, and low GlcN typical of HS; group B had high GlcN and GlcNS, and low GlcNAc; group C had high di- and trisulfated, and low unsulfated and monosulfated disaccharide repeats. These batches could be placed in the same categories based on their 1H NMR spectra and molecular weights. Anticoagulant and growth factor binding activities of these HS batches did not fit within these same groups but were related to the proportions of more highly sulfated disaccharide repeats.

History

Preferred citation

Sargison, L., Smith, R. A. A., Carnachan, S. M., Daines, A. M., Brackovic, A., Kidgell, J. T., Nurcombe, V., Cool, S. M., Sims, I. M. & Hinkley, S. F. R. (2022). Variability in the composition of porcine mucosal heparan sulfates. Carbohydrate Polymers, 282, 119081-119081. https://doi.org/10.1016/j.carbpol.2021.119081

Journal title

Carbohydrate Polymers

Volume

282

Publication date

2022-04-15

Pagination

119081-119081

Publisher

Elsevier BV

Publication status

Published

Online publication date

2022-01-04

ISSN

0144-8617

eISSN

1879-1344

Article number

119081

Language

en