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Type II-activated murine macrophages produce IL-4

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posted on 2022-11-24, 23:17 authored by Anne La FlammeAnne La Flamme, M Kharkrang, S Stone, S Mirmoeini, D Chuluundorj, R Kyle
Background: Type II activation of macrophages is known to support Th2 responses development; however, the role of Th2 cytokines (esp. IL-4) on type II activation is unknown. To assess whether the central Th2 cytokine IL-4 can alter type II activation of macrophages, we compared the ability of bone marrow-derived macrophages from wild type (WT) and IL-4Rα-deficient mice to be classically or type II-activated in vitro. Results: We found that although both WT and IL-4Rα-deficient macrophages could be classically activated by LPS or type II activated by immune complexes plus LPS, IL-4Rα-deficient macrophages consistently produced much higher levels of IL-12p40 and IL-10 than WT macrophages. Additionally, we discovered that type II macrophages from both strains were capable of producing IL-4; however, this IL-4 was not responsible for the reduced IL-12p40 and IL-10 levels produced by WT mice. Instead, we found that derivation culture conditions (GM-CSF plus IL-3 versus M-CSF) could explain the different responses of BALB/c and IL-4Rα-/- macrophages, and these cytokines shaped the ensuing macrophage such that GM-CSF plus IL-3 promoted more IL-12 and IL-4 while M-CSF led to higher IL-10 production. Finally, we found that enhanced IL-4 production is characteristic of the type II activation state as other type II-activating products showed similar results. Conclusions: Taken together, these results implicate type II activated macrophages as an important innate immune source of IL-4 that may play an important role in shaping adaptive immune responses. © 2012 La Flamme et al.

History

Preferred citation

La Flamme, A., Kharkrang, M., Stone, S., Mirmoeini, S., Chuluundorj, D. & Kyle, R. (2012). Type II-activated murine macrophages produce IL-4. PLoS ONE, 7(10). https://doi.org/10.1371/journal.pone.0046989

Journal title

PLoS ONE

Volume

7

Issue

10

Publication date

2012-01-01

Pagination

(9)

Publisher

Public Library of Science (PLoS)

Publication status

Published

Contribution type

Article

Online publication date

2012-10-05

ISSN

1932-6203

eISSN

1932-6203

Article number

e4698

Language

en