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The Fatty Acid Methyl Ester (FAME) profile of Phytophthora agathidicida and its potential use as diagnostic tool

journal contribution
posted on 2024-04-25, 04:01 authored by Randy Lacey, BA Sullivan-Hill, Julie DeslippeJulie Deslippe, Robert KeyzersRobert Keyzers, Monica GerthMonica Gerth
Phytophthora diseases cause devastation to crops and native ecosystems worldwide. In New Zealand, Phytophthora agathidicida is threatening the survival of kauri, an endemic, culturally and ecologically important tree species. The current method for detecting P. agathidicida is a soil bating assay that is time-consuming and requires high levels of expertise to assess, thus limiting the analytical sample throughput. Here, we characterized the fatty acid methyl ester (FAME) profile of P. agathidicida. We also compared it with the FAME profile of P. cinnamomi and assessed the efficacy of FAME analysis as a diagnostic tool for detecting the pathogen in soil samples. In FAME analysis, the total fatty acid content is isolated from a sample and converted to FAMEs for analysis, a process that takes less than a day. Unique fatty acid acyl chains can serve as biomarkers for specific organisms. We detected 12 fatty acids in P. agathidicida, two of which (20:4ω6 and 20:5ω3) show promise as potential Phytophthora specific biomarkers. Collectively, these findings advance our fundamental understanding of P. agathidicida biology and provide a promising technique to increase the rate of sample processing and the speed of pathogen detection for P. agathidicida in soil.

History

Preferred citation

Lacey, R. F., Sullivan-Hill, B. A., Deslippe, J. R., Keyzers, R. A. & Gerth, M. L. (2021). The Fatty Acid Methyl Ester (FAME) profile of Phytophthora agathidicida and its potential use as diagnostic tool. FEMS Microbiology Letters, 368(17), fnab113--. https://doi.org/10.1093/femsle/fnab113

Journal title

FEMS Microbiology Letters

Volume

368

Issue

17

Publication date

2021-09-01

Pagination

fnab113-

Publisher

Oxford University Press (OUP)

Publication status

Published

Online publication date

2021-08-27

ISSN

0378-1097

eISSN

1574-6968

Article number

ARTN fnab113

Language

en