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Synthesis of 13 C-labelled sulfated N-acetyl-d-lactosamines to aid in the diagnosis of mucopolysaccharidosis diseases

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Morquio A syndrome is an autosomal mucopolysaccharide storage disorder that leads to accumulation of keratan sulfate. Diagnosis of this disease can be aided by measuring the levels of keratan sulfate in the urine. This requires the liquid chromatography tandem mass spectrometry (LCMS/MS) measurement of sulfated N-acetyl-d-lactosamines in the urine after cleavage of the keratan sulfate with keratanase II. Quantification requires isotopically-labelled internal standards. The synthesis of these 13 C 6 -labelled standards from 13 C 6 -galactose and N-acetylglucosamine is described. The required protected disaccharide is prepared utilising a regioselective, high yielding β-galactosylation of a partially protected glucosamine acceptor and an inverse addition protocol. Subsequent synthesis of the 13 C 6 -labelled mono and disulfated N-acetyllactosamines was achieved in five and eight steps, respectively, from this intermediate to provide internal standards for the LCMS/MS quantification of keratan sulfate in urine.

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Preferred citation

Cameron, S. A., Zubkova, O. V., Toms, S., Furneaux, R. H. & Rendle, P. M. (2019). Synthesis of 13 C-labelled sulfated N-acetyl-d-lactosamines to aid in the diagnosis of mucopolysaccharidosis diseases. Journal of Labelled Compounds and Radiopharmaceuticals, 62(2), 67-76. https://doi.org/10.1002/jlcr.3697

Journal title

Journal of Labelled Compounds and Radiopharmaceuticals

Volume

62

Issue

2

Publication date

2019-02-01

Pagination

67-76

Publisher

Wiley

Publication status

Published

Online publication date

2018-12-11

ISSN

0362-4803

eISSN

1099-1344

Language

en