Shu Hui's virus torture paper as submitted 9Jun17.pdf (1.87 MB)

Low root-to-root transmission of a tobamovirus, yellow tailflower mild mottle virus, and resilience of its virions

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journal contribution
posted on 10.08.2021, 01:06 by SH Koh, H Li, K Sivasithamparam, Ryan AdmiraalRyan Admiraal, MGK Jones, SJ Wylie
Tobamoviruses are serious pathogens because they have extremely stable virions, they are transmitted by contact, and they often induce severe disease in crops. Knowledge of the routes of transmission and resilience of tobamovirus virions is essential in understanding the epidemiology of this group of viruses. Here, an isolate of the tobamovirus yellow tailflower mild mottle virus (YTMMV) was used to examine root-to-root transmission in soil and in a hydroponic growth environment. Root-to-root transmission occurred rarely, and when it occurred plants did not exhibit systemic movement of the virus from the roots to the shoots over a 30-day period. The resilience of YTMMV virions was tested in dried leaf tissue over time periods from one hour to one year under temperatures ranging from −80 to 160 °C. Infectivity was maintained for at least a year when incubated at −80 or 22 °C, or at fluctuating ambient temperatures of 0.8 to 44.4 °C, but incubation under dry conditions at 160 °C for >4 days eliminated infectivity. Exposure of virions to 0.1 m sodium hydroxide or 20% w/v skimmed milk solution for 30 min, treatments recommended for tobamovirus inactivation, did not abolish infectivity of YTMMV.

History

Preferred citation

Koh, S. H., Li, H., Sivasithamparam, K., Admiraal, R., Jones, M. G. K. & Wylie, S. J. (2018). Low root-to-root transmission of a tobamovirus, yellow tailflower mild mottle virus, and resilience of its virions. Plant Pathology, 67(3), 651-659. https://doi.org/10.1111/ppa.12758

Journal title

Plant Pathology

Volume

67

Issue

3

Publication date

01/04/2018

Pagination

651-659

Publisher

Wiley

Publication status

Published

Online publication date

23/10/2017

ISSN

0032-0862

eISSN

1365-3059

Language

en