Open Access Te Herenga Waka-Victoria University of Wellington
Browse
Kihika cryoprotecion dinoflagellates.pdf (1.51 MB)
Download file

Cryoprotectant treatment tests on three morphologically diverse marine dinoflagellates and the cryopreservation of Breviolum sp. (Symbiodiniaceae)

Download (1.51 MB)
journal contribution
posted on 2022-02-09, 19:47 authored by Joseph KihikaJoseph Kihika, SA Wood, L Rhodes, KF Smith, L Thompson, S Challenger, Kenneth RyanKenneth Ryan
Dinoflagellates are among the most diverse group of microalgae. Many dinoflagellate species have been isolated and cultured, and these are used for scientific, industrial, pharmaceutical, and agricultural applications. Maintaining cultures is time-consuming, expensive, and there is a risk of contamination or genetic drift. Cryopreservation offers an efficient means for their long-term preservation. Cryopreservation of larger dinoflagellate species is challenging and to date there has been only limited success. In this study, we explored the effect of cryoprotectant agents (CPAs) and freezing methods on three species: Vulcanodinium rugosum, Alexandrium pacificum and Breviolum sp. A total of 12 CPAs were assessed at concentrations between 5 and 15%, as well as in combination with dimethyl sulfoxide (DMSO) and other non-penetrating CPAs. Two freezing techniques were employed: rapid freezing and controlled-rate freezing. Breviolum sp. was successfully cryopreserved using 15% DMSO. Despite exploring different CPAs and optimizing the freezing techniques, we were unable to successfully cryopreserve V. rugosum and A. pacificum. For Breviolum sp. there was higher cell viability (45.4 ± 2.2%) when using the controlled-rate freezing compared to the rapid freezing technique (10.0 ± 2.8%). This optimized cryopreservation protocol will be of benefit for the cryopreservation of other species from the family Symbiodiniaceae.

History

Preferred citation

Kihika, J. K., Wood, S. A., Rhodes, L., Smith, K. F., Thompson, L., Challenger, S. & Ryan, K. G. (2022). Cryoprotectant treatment tests on three morphologically diverse marine dinoflagellates and the cryopreservation of Breviolum sp. (Symbiodiniaceae). Scientific Reports, 12(1), 646-. https://doi.org/10.1038/s41598-021-04227-2

Journal title

Scientific Reports

Volume

12

Issue

1

Publication date

2022-12-01

Pagination

646

Publisher

Springer Science and Business Media LLC

Publication status

Accepted

Online publication date

2022-01-13

ISSN

2045-2322

eISSN

2045-2322

Article number

646

Language

en

Usage metrics

    Journal articles

    Exports