20 Comoletti Neuropharm NLGN review.pdf (9.15 MB)
Download file

Comparative mapping of selected structural determinants on the extracellular domains of cholinesterase-like cell-adhesion molecules

Download (9.15 MB)
journal contribution
posted on 04.08.2022, 22:12 authored by Davide ComolettiDavide Comoletti, L Trobiani, A Chatonnet, Y Bourne, P Marchot
Cell adhesion generally involves formation of homophilic or heterophilic protein complexes between two cells to form transcellular junctions. Neural cell-adhesion members of the α/β-hydrolase fold superfamily of proteins use their extracellular or soluble cholinesterase-like domain to bind cognate partners across cell membranes, as illustrated by the neuroligins. These cell-adhesion molecules currently comprise the synaptic organizers neuroligins found in all animal phyla, along with three proteins found only in invertebrates: the guidance molecule neurotactin, the glia-specific gliotactin, and the basement membrane protein glutactin. Although these proteins share a cholinesterase-like fold, they lack one or more residues composing the catalytic triad responsible for the enzymatic activity of the cholinesterases. Conversely, they are found in various subcellular localisations and display specific disulfide bonding and N-glycosylation patterns, along with individual surface determinants possibly associated with recognition and binding of protein partners. Formation of non-covalent dimers typical of the cholinesterases is documented for mammalian neuroligins, yet whether invertebrate neuroligins and their neurotactin, gliotactin and glutactin relatives also form dimers in physiological conditions is unknown. Here we provide a brief overview of the localization, function, evolution, and conserved versus individual structural determinants of these cholinesterase-like cell-adhesion proteins. This article is part of the special issue entitled ‘Acetylcholinesterase Inhibitors: From Bench to Bedside to Battlefield’.

History

Preferred citation

Comoletti, D., Trobiani, L., Chatonnet, A., Bourne, Y. & Marchot, P. (2021). Comparative mapping of selected structural determinants on the extracellular domains of cholinesterase-like cell-adhesion molecules. Neuropharmacology, 184, 108381-108381. https://doi.org/10.1016/j.neuropharm.2020.108381

Journal title

Neuropharmacology

Volume

184

Publication date

15/02/2021

Pagination

108381-108381

Publisher

Elsevier BV

Publication status

Published

Online publication date

06/11/2020

ISSN

0028-3908

eISSN

1873-7064

Article number

108381

Language

en