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A comparison between oestradiol aptamers as receptors in CNT FET biosensors

journal contribution
posted on 2024-01-24, 04:59 authored by E Cassie, H Dunham, E Happe, HPT Nguyen, Janet PitmanJanet Pitman, Natalie Plank
Point of care tests for measuring the concentration of small molecules such as oestradiol are both highly desirable for healthcare and challenging to design. Field effect transistors functionalised with DNA aptamers, FET aptasensors, are a promising candidate for such tests, however, the performance of FET aptasensors does not consistently keep pace with the performance of particular aptamers in isolation. To better understand the cause of this discrepancy, we compare the performance of two oestradiol aptamers in carbon nanotube network FET aptasensors, and further characterise these aptamers using circular dichroism spectroscopy. We show that both aptamers work effectively as sensors at a much higher analyte concentration, 10−6 M, than would be predicted by published Kd values, approximately 10−8 M. We show qualitatively different behaviour between otherwise identical sensors based on the aptamer they are functionalised with, at analyte concentrations well below the limit of detection. Our results suggest that the discrepancy between predicted and realised performance of carbon nanotube network FET aptasensors has two contributing factors: the difference between the ionic environment used for sensing and the environment the aptamers have been characterised in, and the use of SELEX methods that produce aptamers with minimal structure shift on binding. To optimise the sensing response from FET aptasensors, aptamers should be selected for large structure shifts upon binding, and so that they exhibit strong binding in the ionic environment that will be used for sensing.

History

Preferred citation

Cassie, E., Dunham, H., Happe, E., Nguyen, H. P. T., Pitman, J. L. & Plank, N. O. V. (2023). A comparison between oestradiol aptamers as receptors in CNT FET biosensors. Sensors and Diagnostics, 2(6), 1561-1573. https://doi.org/10.1039/d3sd00055a

Journal title

Sensors and Diagnostics

Volume

2

Issue

6

Publication date

2023-10-02

Pagination

1561-1573

Publisher

Royal Society of Chemistry (RSC)

Publication status

Published

Online publication date

2023-01-01

ISSN

2635-0998

eISSN

2635-0998

Language

en

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